Journal: Stroke

Article Title: Neuronal CD200 Signaling Is Protective in the Acute Phase of Ischemic Stroke.

doi: 10.1161/STROKEAHA.120.032374

Figure Lengend Snippet: Figure 1. Effects of neuronal CD200 signaling on stroke outcomes and neuronal apoptosis at 3 days after stroke. (A) Representative images of brain slices stained with cresyl violet (CV) and quantification of brain infarct volumes, NDS, and corner test scores in TMX vs. VEH mice. (B) TUNEL labelled cell apoptosis in peri-infarct area of TMX and VEH treated mice and the quantification of percent of TUNEL positive cells in ipsilateral hemispheres Each dot represents the average of % apoptotic cells from eight 20× fields/animal in the peri-infarct area. N=3 for sham and 6–7 for stroke per group; *P < 0.05.

Article Snippet: Determination of CD200 and cytokine Levels The levels of CD200 in mouse brain, plasma and in the supernatants of primary neuronal culture were measured by a sandwich ELISA kit according to manufacture instruction ((# EK1185, Mouse CD200 PicoKine ELISA Kit, Boster Biological, Pleasanton, CA, USA.).

Techniques: Staining, TUNEL Assay

Journal: Stroke

Article Title: Neuronal CD200 Signaling Is Protective in the Acute Phase of Ischemic Stroke.

doi: 10.1161/STROKEAHA.120.032374

Figure Lengend Snippet: Figure 4. Plasma cytokine levels after ischemic injury. Pro-inflammatory (TNFα, IL-1β and IL-6) and anti-inflammatory (IL-10 and IL-4) cytokine levels (pg/mL) were measured in the plasma of VEH and TMX treated sham/stroke mice at 3d (A) and 7d (B). N=5–6 sham and 6–7 stroke animals per group; *P < 0.05. (C) CD200 levels in naïve brains (left), in the plasma (middle) of TMX and VEH treated mice, and in neuronal culture medium after OGD (right). N=5–6 sham and 6–8 stroke animals per group; *P < 0.05. For neuronal culture medium, N=4 independent OGD experiments; *P < 0.05.

Article Snippet: Determination of CD200 and cytokine Levels The levels of CD200 in mouse brain, plasma and in the supernatants of primary neuronal culture were measured by a sandwich ELISA kit according to manufacture instruction ((# EK1185, Mouse CD200 PicoKine ELISA Kit, Boster Biological, Pleasanton, CA, USA.).

Techniques: Clinical Proteomics

Journal: Stroke

Article Title: Neuronal CD200 Signaling Is Protective in the Acute Phase of Ischemic Stroke.

doi: 10.1161/STROKEAHA.120.032374

Figure Lengend Snippet: Figure 5. Stroke outcomes and flow cytometry in CD200 Fc treated mice. (A) Representative images of brain slices stained with cresyl violet (CV) and quantification of brain infarct volumes, NDS, and corner test scores in CD200Fc vs. IgG treated mice. N=3 for sham and 6–7 for stroke per group; *P < 0.05. (B) Microglial expression of cell membrane markers CD68/CD206 measured as mean fluorescence intensity (MFI). (C) Microglial expression of intracellular markers IL-1β/TNFα. For (B) and (C), n=4/sham group and n=6/stroke group; *P < 0.05.

Article Snippet: Determination of CD200 and cytokine Levels The levels of CD200 in mouse brain, plasma and in the supernatants of primary neuronal culture were measured by a sandwich ELISA kit according to manufacture instruction ((# EK1185, Mouse CD200 PicoKine ELISA Kit, Boster Biological, Pleasanton, CA, USA.).

Techniques: Flow Cytometry, Staining, Expressing, Membrane, Fluorescence

Journal: Stroke

Article Title: Neuronal CD200 Signaling Is Protective in the Acute Phase of Ischemic Stroke.

doi: 10.1161/STROKEAHA.120.032374

Figure Lengend Snippet: Figure 6. Interaction of neuronal CD200 with lymphocytic CD200R1. (A) The purity of CD45+CD3+

Article Snippet: Determination of CD200 and cytokine Levels The levels of CD200 in mouse brain, plasma and in the supernatants of primary neuronal culture were measured by a sandwich ELISA kit according to manufacture instruction ((# EK1185, Mouse CD200 PicoKine ELISA Kit, Boster Biological, Pleasanton, CA, USA.).

Techniques: